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Fluorescence gel retardation assay to detect protein-protein interactions

Sang-Hyun Park1, Ronald T Raines

  • 1Department of Cellular and Molecular Pharmacology, University of California-San Francisco, San Francisco, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 6, 2004
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...