Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Overview of Hematopoiesis

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...

Production of Formed Elements

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[A case of thoracic aortic aneurysm after coronary artery bypass grafting was treated with integrative branch stent combined with extracorporeal fenestration technique and internal branch technique].

Zhonghua xin xue guan bing za zhi·2025

Same author

[Clinicopathological analysis of 2 cases of primary dedifferentiated liposarcoma of the heart].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025

Same author

[One case of acute Stanford B aortic dissection complicated with vagus right subclavian artery treated with integrated branching stent combined with periscope technique].

Zhonghua xin xue guan bing za zhi·2025

Same author

A risk-based stochastic energy-water-carbon nexus analytical model to support provincial multi-system synergistic management - A case study of Shanxi, China.

The Science of the total environment·2024

Same author

[Analysis of pollution status and influencing factors of polybrominated diphenyl ethers in household dust in five cities in northern China].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024

Same author

[Mucoepidermoid carcinoma of the stomach: report of two cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2024

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Published on: March 21, 2017

Microenvironment-driven changes in the expression profile of hematopoietic cobblestone area-forming cells.

M L Choong¹, B Luo, H F Lodish

¹Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, Centros, Singapore, Republic of Singapore. choong_meng_ling@bti.a-star.edu.sg

Annals of Hematology

|April 6, 2004

Summary

Primitive hematopoietic stem cells reside in bone marrow adherent layers, confirmed by gene expression analysis. These cells exhibit homing and adhesion properties, unlike more differentiated free-floating cells.

More Related Videos

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Published on: September 28, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Published on: March 21, 2017

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Published on: September 28, 2022

Area of Science:

Hematology
Stem Cell Biology
Molecular Biology

Background:

Ex vivo bone marrow cultures highlight the adherent layer as a reservoir for primitive hematopoietic stem cells.
Derivative stem cells and differentiated progenitors are continuously generated from this adherent layer.

Purpose of the Study:

To analyze and compare mRNA expression profiles between hematopoietic progenitor cells in cobblestone areas (CA) and free-floating cells.
To elucidate the molecular differences underlying stem cell hierarchy and differentiation within bone marrow niches.

Main Methods:

Utilized Affymetrix GeneChip for mRNA expression analysis.
Compared gene expression between cobblestone area-forming cells (CAFC) and free-floating cells from mouse bone marrow progenitor cell line FDCP-Mix and S17 stromal cells.

Main Results:

Identified 29 genes with >5-fold higher expression in CAFC and 55 genes in supernatant cells.
CAFC expressed genes related to homing, adhesion, and suppressed differentiation, indicating primitive nature.
Free-floating cells showed mature lineage and differentiation-specific genes.

Conclusions:

Gene expression analysis confirms the primitive nature of hematopoietic cells within the bone marrow adherent layer (CAFC).
Discovered significant expression of secreted and surface proteins in CA hematopoietic cells.
Suggests complex interactions involving hematopoietic cells, stromal cells, and extracellular matrix drive stem cell growth and differentiation.