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Solubilization of dentin matrix collagen in situ
A J Van Strijp1, B Klont, J M Ten Cate
1Department of Cariology and Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands.
Journal of Dental Research
|August 1, 1992
Summary
The oral environment significantly degrades dentin collagen, causing substantial collagen loss and structural damage. Microorganisms in the mouth contribute to this breakdown, affecting dental prostheses.
Area of Science:
- Biomaterials Science
- Oral Biology
- Dental Materials
Background:
- Dentin matrix collagen is crucial for tooth structure and integrity.
- Understanding the oral environment's impact on collagen is vital for dental material longevity.
Purpose of the Study:
- To investigate the degradation of dentin matrix collagen in the oral environment.
- To quantify collagen loss and assess structural changes in demineralized dentin specimens.
Main Methods:
- Demineralized dentin specimens were exposed intra-orally for seven weeks.
- Collagen degradation was assessed using trypsin and bacterial collagenase.
- Microscopic analysis (light and transmission electron microscopy) evaluated structural integrity.
Main Results:
- Intra-oral exposure resulted in collagen loss ranging from 1 to 47 wt%.
- A significant portion of denatured collagen was rapidly solubilized.
- Microscopic examination revealed surface degradation and microbial invasion of dentin tubules.
Conclusions:
- The oral environment poses a significant risk to dentin matrix collagen integrity.
- Proteolytic activity of oral microflora is a key factor in collagen degradation.
- Dentin degradation can lead to structural compromise and enlarged tubules, impacting dental restorations.