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Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
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Efficient method for mapping and characterizing structures of deletion mutations in gpt delta mice using Southern

Atsushi Shibata1, Mitsuko Masutani, Nobuo Kamada

  • 1Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.

Environmental and Molecular Mutagenesis
|April 6, 2004
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...