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Related Experiment Videos

Novel pH-sensitive paramagnetic liposomes with improved MR properties.

Knut-Egil Løkling1, Roald Skurtveit, Atle Bjørnerud

  • 1Department of Medicinal Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway. knut-egil.loking@amersham.com

Magnetic Resonance in Medicine
|April 6, 2004
PubMed
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New pH-sensitive liposomes offer improved stability for MRI monitoring of pathologic pH changes. The dipalmitoylglycerosuccinate (DPSG)-based system demonstrates stability in blood and selective pH sensitivity, showing promise for in vitro diagnostic applications.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Magnetic Resonance Imaging

Background:

  • Paramagnetic pH-sensitive liposomes are a novel approach for MRI-based monitoring of pathological pH variations.
  • Essential liposome characteristics include blood stability and selective release of encapsulated agents at lower tissue pH.

Purpose of the Study:

  • To formulate and characterize novel liposomal systems for pH monitoring using MRI.
  • To enhance the stability of existing pH-sensitive liposomes in blood while maintaining pH responsiveness.

Main Methods:

  • Liposomal systems were formulated using dipalmitoylphosphatidylethanolamine (DPPE) with different amphiphiles (palmitic acid (PA), dipalmitoylglycerosuccinate (DPSG)).
  • Characterization involved relaxometry, cryo-transmission electron microscopy (cryo-TEM), and MRI.

Related Experiment Videos

  • Stability was assessed in blood, and pH sensitivity was evaluated in buffer and in vitro MRI phantom studies.

    • Main Results:

    • Cholesterol incorporation into DPPE/PA liposomes improved blood stability but reduced pH sensitivity.
    • Polyethylene glycol (PEG)-modified DPPE/PA liposomes were pH-insensitive and unstable in blood.
    • DPPE/DPSG liposomal GdDTPA-BMA exhibited stability in blood at physiological pH and marked pH sensitivity, with retained sensitivity after blood preincubation.

    Conclusions:

    • DPPE/DPSG liposomal GdDTPA-BMA represents an improved system for pH-sensitive MRI contrast agents.
    • This formulation demonstrates superior stability and pH responsiveness compared to previous systems.
    • The study validates the potential of DPPE/DPSG liposomes for in vitro pH monitoring applications via MRI.