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Updated: Aug 25, 2026
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
Published on: April 9, 2015
[Pilot study of neonatal rat cardiac myocytes cultured for three-dimensional modeling in simulated microgravity]
Fen Yang1, Ying-hui Li, Jie-lin Nie
1Institute of Space Medico-Engineering, Beijing, P. R. China 100094.
Objective:
To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity.
Methods:
Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirred in spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed under inverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay.
Results:
Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLA-cardiac myocytes was found in some areas.
Conclusion:
The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.
