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Electrophoretically mediated microanalysis
Sona Nováková1, Sigrid Van Dyck, Ann Van Schepdael
1Laboratory for Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, K. U. Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium.
Journal of Chromatography. A
|April 7, 2004
Summary
This review covers developments in electrophoretically mediated microanalysis (EMMA), a capillary electrophoresis technique for studying enzyme reactions in-line. It details EMMA methods, applications, and highlights EMMA on CE microchip technology.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Microfluidics
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- In-line analysis of enzyme reactions requires specialized methodologies.
- Electrophoretically mediated microanalysis (EMMA) offers a solution for real-time enzymatic studies.
Purpose of the Study:
- To review the current state of electrophoretically mediated microanalysis (EMMA).
- To discuss the principles, procedures, and mathematical aspects of EMMA.
- To highlight applications in enzyme assays and non-enzymatic determinations, including microchip-based systems.
Main Methods:
- Detailed description of EMMA methodology within capillary electrophoresis.
- Explanation of different EMMA modes and operational procedures.
- Focus on EMMA implemented on CE microchips.
Main Results:
- Summarized applications of EMMA for enzyme assays and non-enzymatic analyses in two tables.
- Illustrated the basic principles and mathematical treatments of EMMA.
- Presented EMMA on CE microchip as a significant advancement.
Conclusions:
- EMMA is a versatile microanalytical technique for in-line enzyme reaction studies.
- EMMA on CE microchip represents a novel and promising development in the field.
- The review provides a comprehensive overview of EMMA's current status and future potential.