D609 induces vascular endothelial cells and marrow stromal cells differentiation into neuron-like cells
Nan Wang1, Chun-qing Du, Shao-shan Wang
1Institute of Developmental Biology, School of Life Science, Shandong University, Ji-nan 250100, China.
Aim:
To investigate the effect of tricyclodecane-9-yl-xanthogenate (D609) on cell differentiation in vascular endothelial cells (VECs) and marrow stromal cells (MSCs).
Methods:
Morphological changes were observed under phase contrast microscope. Electron microscope and immunostaining were used for VECs identification. The expressions of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were examined by immunohistochemistry.
Results:
After 6 h of induction with D609, some VECs showed morphological changes characteristic of neurones. 9 h later, more VECs became neuron-like cells. About 30.8% of VECs displayed positive NSE (P<0.01), while the expression of GFAP was negative. When MSCs were exposed to D609, the cells displayed neuronal morphologies, such as pyramidal cell bodies and processes formed extensive networks at 3 h. 6 h later, almost all of the cells exhibited a typical neuronal appearance, and 85.6% of MSCs displayed intensive positive NSE, but GFAP did not express.
Conclusion:
D609 induces VECs and MSCs differentiation into neuron-like cells.
