Killing of Bacillus subtilis spores by a modified Fenton reagent containing CuCl2 and ascorbic acid
Michael P Shapiro1, Barbara Setlow, Peter Setlow
1Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Applied and Environmental Microbiology
|April 7, 2004
Abstract:
Bacillus subtilis spores were killed by CuCl(2)-ascorbic acid, chloride ions were essential for killing of spores, and spores with defective coats were killed more rapidly. CuCl(2)-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl(2)-ascorbic acid may have damage to their inner membrane.
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