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EST-derived microsatellites from Actinidia species and their potential for mapping
L G Fraser1, C F Harvey, R N Crowhurst
1The Horticulture and Food Research Institute of New Zealand, 120 Mt Albert Road, Auckland, New Zealand. lfraser@hortresearch.co.nz
Summary
This study developed cost-effective, highly polymorphic expressed sequence tag-derived microsatellites for kiwifruit genetic mapping. These markers accelerate breeding and functional genomics in Actinidia species.
Area of Science:
- Plant Genetics
- Genomics
- Molecular Biology
Background:
- Developing genetic maps for Actinidia species (kiwifruit) is crucial for breeding and functional genomics.
- Existing methods for genetic map construction can be time-consuming and expensive.
Purpose of the Study:
- To evaluate the frequency and polymorphism of microsatellites derived from expressed sequence tags (ESTs) for efficient kiwifruit genetic mapping.
- To assess the utility of EST-derived microsatellites for accelerating kiwifruit breeding programs.
Main Methods:
- Microsatellites were sampled from ESTs in Actinidia species, focusing on perfect dinucleotide repeats.
- 150 microsatellites with >10 repeat units were selected and amplified in an intraspecific cross of Actinidia chinensis.
- Linkage analysis was performed using Joinmap software with a LOD score of 3.
Main Results:
- 93.5% of amplified microsatellites were polymorphic and segregated in the mapping population.
- EST-derived microsatellites provided a high number of markers rapidly and cost-effectively.
- Markers showed random distribution across linkage groups, with some mapping to separate groups.
Conclusions:
- EST-derived microsatellites are a valuable tool for rapid and cost-effective genetic map construction in Actinidia.
- These markers are highly polymorphic and informative, enhancing the value of genomic maps with functional information.
- The study demonstrates the potential of EST-microsatellites for advancing kiwifruit breeding and functional genomics.
