Simultaneous micellar liquid chromatographic analysis of seven water-soluble vitamins: optimization using...

Area of Science:

• Analytical Chemistry
• Chromatography
• Pharmaceutical Analysis

Background:

• Accurate quantification of water-soluble vitamins in pharmaceutical formulations is crucial for quality control.
• Micellar liquid chromatography (MLC) offers a versatile platform for separating complex mixtures.
• Optimization of mobile phase composition is key to achieving efficient separations in MLC.

Purpose of the Study:

• To develop and optimize a gradient micellar liquid chromatography method for the simultaneous determination of seven water-soluble vitamins.
• To apply a super-modified simplex (SMS) method for efficient mobile phase optimization.
• To validate the analytical performance of the developed method for multivitamin tablet analysis.

Main Methods:

• Gradient micellar liquid chromatography (MLC) with UV detection.
• Super-modified simplex (SMS) optimization of mobile phase parameters (SDS concentration, pH, organic modifier percentage).
• Investigation of column temperature and organic modifier type (1-butanol).

Main Results:

• Optimized mobile phase: 16 mM sodium dodecyl sulfate (SDS), 0.02 M phosphate buffer (pH 3.6), and a 3.5-10% (v/v) 1-butanol gradient.
• Optimal column temperature: 35°C.
• Method demonstrated excellent linearity (r>0.9970), acceptable precision (RSD <8.90%), and accuracy (88-103% recovery).

Conclusions:

• The SMS-optimized gradient MLC method provides a robust and efficient approach for the determination of water-soluble vitamins in multivitamin tablets.
• The developed method meets analytical requirements for linearity, precision, and accuracy.
• This technique is suitable for routine quality control analysis of vitamin supplements.