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Glomerular expression of platelet-derived growth factor (PDGF)-A, -B chain and PDGF receptor-alpha, -beta in human

Goro Uehara1, Daisuke Suzuki, Masao Toyoda

  • 1Division of Nephrology and Metabolism, Department of Internal Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, 259-1193, Japan.

Clinical and Experimental Nephrology
|April 7, 2004
PubMed
Abstract

Insights

Increased expression of Platelet-Derived Growth Factor-B (PDGF-B) and its receptor PDGFR-Beta is linked to early diabetic nephropathy (DN) glomerular lesions. This suggests PDGF-B/PDGFR-Beta plays a key role in the progression of DN.

Area of Science:

  • Nephrology
  • Diabetology
  • Molecular Biology

Background:

  • Diabetic nephropathy (DN) is characterized by mesangial expansion.
  • Platelet-derived growth factor (PDGF) is implicated in extracellular matrix production in renal diseases.

Purpose of the Study:

  • To investigate the expression of PDGF and PDGF receptor (PDGFR) mRNA in renal tissues of type 2 diabetic patients with DN.
  • To correlate PDGF and PDGFR expression with the severity of DN.

Main Methods:

  • Analysis of renal biopsies from 20 type 2 diabetic patients with DN (grades I and II) and 10 normal human kidneys (NHK).
  • Evaluation of PDGF-A, -B, and PDGFR-Alpha, -Beta mRNA expression and localization using in situ hybridization.
  • Quantification of mRNA expression by counting positive cells in nonsclerotic glomeruli.

Main Results:

  • PDGF and PDGFR mRNAs were primarily expressed in glomerular mesangial and epithelial cells.
  • PDGF-A and PDGFR-Alpha mRNA levels were similar in DN and NHK.
  • PDGF-B and PDGFR-Beta mRNA levels were significantly higher in DN patients compared to NHK, and higher in DN grade I than grade II, with PDGF-B correlating with PDGFR-Beta.

Conclusions:

  • PDGF-B and PDGFR-Beta expression is a significant factor in early-stage glomerular lesions of DN.
  • These findings highlight the role of the PDGF-B/PDGFR-Beta pathway in the pathogenesis of DN.

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