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Updated: Aug 25, 2026
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
[Expression of leucocyte cell-surface antigens on colon cancer cell line HR8348]
Shi-long Yang1, Yon-min Tang, Hong-qiang Shen
1The Affiliated Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. yangshilonghz@zju.edu.cn
Objective:
To define the immune phenotype of colon cancer cells.
Methods:
Using a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.
Results:
HR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.
Conclusion:
The results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.
