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Updated: Jun 3, 2026
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
Published on: September 3, 2012
Evidence of metyrapone reduction by two Mycobacterium strains shown by 1H NMR
Bruno Combourieu1, Pascale Besse, Martine Sancelme
1Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 du CNRS, Université, Blaise Pascal, 63177 Aubière Cedex, France.
Abstract:
In situ 1H NMR monitoring of metyrapone incubations with resting-cells of two strains of Mycobacterium, Mycobacterium aurum MO1 and Mycobacterium sp. RP1, showed the biotransformation of this compound, and more precisely the carbonyl-reduction of metyrapone into the corresponding alcohol, metyrapol. This reduction produced both enantiomers. The use of inhibitors allowed us to show the multiple enzymatic activities involved in this biotransformation including carbonyl reductase (EC 1.1.1.1.84) from the short-chain dehydrogenase superfamily and aldehyde reductase (EC 1.1.1.2) from the aldo-keto reductase superfamily.
