New glucose biosensor based on glucose oxidase-immobilized gelatin film coated electrodes

In this article we describe the preparation of a new glucose biosensor based on electrode coating with glucose oxidase-immobilized gelatin. Glucose oxidase was immobilized onto gelatin by cross-linking with chromium III acetate. Properties of the immobilized glucose oxidase enzyme electrode have been studied and results were compared with free enzyme electrode. Effect of temperature and pH variations, kinetic properties, and re-usability were among the subjects analyzed. Also interfering effect of the electro-active compounds was investigated and their influence on the behavior of glucose oxidase electrode was determined. The response time of the developed glucose oxidase enzyme electrode was 60 s with a detection limit of 0.25 mM and preserved its accuracy within the 2 months period of reuse experiments.