[Flow cytometry dynamic study binding to bromodeoxyuridine in golden hamster cheek pouch premalignancy]

OBJECTIVE:In order to study cellular dynamicfeatures in carcinoma and premalignancy originated inepithelial tissue. METHODS: The golden hamster pouch premalignant model with dimethylbenzanthracence(DMBA) smeared was established by Salley and detected with flow cytometry(FCM) binding to bromodeoxyuridine(BRDU),30 Syrian golden hamsters were grouped and done contrast study. RESULTS: The cellular lesions were aggravated gradually along with extention of smeared DMBA microstopically,FCM detective results demonstrated that the detective rate of Sporiod cell between contrast and smeared group had significant difference. Even though mild dysplsia presented microscopically FCM detective result and significant difference. CONCLUSION: The study suggests that golden hamster cheek pouch premalignancy has cellular dynamicfeatures of dysplasia.