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Published on: March 16, 2012
[Cloning the gene fragment coding the putative integrase-like protein from X maltophilia]
Shu-fang Liang1, Man-ling Chen, Chao-yang Zhai
1Biochemistry Department, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To amplify the nucleotide sequence of CG-like receptor from X anthomonas maltophilia(X maltophilia).
Methods:
Using the specific primer P1 designed by Grover's reported 342 bp partial nucleotide sequence of X maltophilia CG-like receptor and random primer to PCR amplify, PCR product was cloned in the pUCm-T vector. After the recombinant plasmid was tested by restriction endonuclease digestion, the insert on the recombinant plasmid was sequenced and analyzed.
Results:
About 500 bp PCR product was cloned in the pUCm-T vector and obtained the recombinant pUCm-Int. By sequencing to the insert on the pUCm-Int with M13 universal sequencing primers, the 410-486 bp fragment of the cloned 510 bp nucleotide sequence (GenBank accession number: AY363962) showed 84% identity with the 9304-8958 bp fragment of the XACb0009 gene on plasmid pXAC64 of Xanthomonas axonopodis pv. citri. And the 4-166aa fragment of its translated 169aa sequence had 62% identity with the 38-200aa sequence of integrase-like protein coded by the XACb0009 gene.
Conclusion:
The cloned 510 bp nucleotide sequence was possibly the partial gene sequence coding the integrase-like protein of X maltophilia.
Insights
Researchers amplified and sequenced a 510 bp nucleotide sequence from Xanthomonas maltophilia, identifying it as a potential partial gene for integrase-like protein. This finding advances understanding of X. maltophilia genetics.
Area of Science:
- Molecular Biology
- Genetics
- Microbiology
Context:
- Xanthomonas maltophilia is an opportunistic pathogen.
- CG-like receptors play roles in bacterial pathogenesis.
- Understanding bacterial genetic elements is crucial for developing control strategies.
Purpose:
- To amplify and characterize the nucleotide sequence of a CG-like receptor from Xanthomonas maltophilia.
- To identify potential functional genes within the X. maltophilia genome.
Summary:
- A 510 bp nucleotide sequence was amplified from Xanthomonas maltophilia using PCR and cloned into the pUCm-T vector.
- Sequencing revealed 84% identity to a fragment of the XACb0009 gene from Xanthomonas axonopodis pv. citri.
- The translated protein sequence showed 62% identity to an integrase-like protein, suggesting a potential role in X. maltophilia.
Impact:
- Provides a partial nucleotide sequence for a CG-like receptor in X. maltophilia.
- Contributes to the genomic characterization of X. maltophilia.
- Offers insights into potential virulence factors and genetic mechanisms of X. maltophilia.
