Related Experiment Video
Updated: Aug 24, 2026
Model of Ischemia and Reperfusion Injury in Rabbits
Published on: November 3, 2023
[Changes of connexin 43 in rabbit with early myocardial ischemia]
Yang Chen1, Hua-zi Hu, Jia-wen Wu
1Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To reveal gap junction connexin 43 (Cx43) in rabbit with early myocardial ischemia.
Methods:
The left coronary arteries were ligated to create early myocardial ischemia. Cx43 was visualized by use of SP immunohistochemical method (IHC) and Colloidal gold of the electron microscopy, and a comparison was made between the experiment group and the control group. The semi-quantitation assessment was performed.
Results:
The positive areas of Cx43 gap junctions by IHC and Colloidal gold particles of the electron microscopy were found decreased by about 40% at 30 minutes of acute myocardial ischemia. The distributions were not obviously modified.
Conclusion:
The decrease of Cx43 is characterized by the time during myocardial ischemia, and the early stage of acute myocardial ischemia in rat is of positive significance.
