Related Experiment Video
Updated: Aug 19, 2026

In vitro Reconstitution of the Active T. castaneum Telomerase
Published on: July 14, 2011
[Inhibitory effect of isorhmnetin on telomerase activity of HeLa cells]
Chun-lei Yang1, Yi Qu, Zheng-rong Wang
1Department of Cell Biology, Life Science College, Sichun University, Chengdu 610041, China.
Objective:
To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells.
Methods:
The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity.
Results:
The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 micrograms/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase.
Conclusion:
By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.
Related Concept Videos
Telomeres and Telomerase
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Replicative Cell Senescence

