Related Experiment Video
Updated: Aug 24, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
[Study on the genetic polymorphisms of D8S1132 locus in Chinese Han, Mongolian and Thai populations]
Objective:
The aim of the present study was to obtain the population genetic data of short tandem repeat (STR) locus D8S1132 and study the difference of allelic frequency distributions among different populations.
Methods:
A sample of 380 unrelated individuals from 3 populations (Han, Mongolian, Thai) were studied using single PCR followed by polyacrylamide gel electrophoresis and silver staining.
Results:
Eleven allels and 42 genotypes were observed. The observed heterozygosity and the discrimination power were 85.0%-89.5% and 94.1%-95.5%, respectively. The genotype distributions of D8S1132 locus in 3 populations were in accordance with Hardy-Weinberg equilibrium and the difference of allelic frequency distributions among 3 populations was obvious.
Conclusion:
The methods established are easy-to-perform and sensitive. The discrimination power and exclusion chances of this locus are desirable for forensic analysis.
Related Concept Videos
Single Nucleotide Polymorphisms-SNPs
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Incomplete Dominance
