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Updated: Aug 24, 2026
A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection
Published on: February 2, 2022
[A new rat model of transplant kidney-sclerosis accelerated by prolonged cold ischemia time]
Yu-peng Xin1, Yi-ping Lu, Xiu-hui Zhang
1Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To establish an accelerated animal model of the chronic renal allograft dysfunction in rat.
Methods:
Kidney transplantation was performed from SD to Wistar strain (allogeneic) according to the procedure of Kamada with some modification. Before the transplantation, the kidney was preserved in 0-4 degrees C heparin sodium chloride solution for prolonging the one hour. The serum creatinine level and pathological change of transplant kidney were observed in the 2nd, 4th, 6th, 8th and 12th weeks post-transplantation.
Results:
After transplantation, the serum creatinine level of recipient rats obviously increased in the 6th week and the pathologic changes of chronic nephropathy evidently appeared in the 8th week when compared to those of the control group with non-reinforcement I/R injury; a statistically significant difference was noted.
Conclusion:
It is simple and feasible to establish a rat model of (SD-->Wistar) transplant kidney-sclerosis accelerated by prolonging I/R injury.
