Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Beta cell differentiation during early human pancreas development.

K Piper1, S Brickwood, L W Turnpenny

  • 1Division of Human Genetics, University of Southampton, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK.

The Journal of Endocrinology
|April 10, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Concordance between a gastrointestinal consultant radiologist, a consultant radiologist and qualified reporting radiographers interpreting abdominal radiographs.

Radiography (London, England : 1995)·2023
Same author

A pilot study to assess radiographer preliminary clinical evaluation (PCE) introduced for emergency department adult appendicular X-ray examinations: Comparison of trained and untrained radiographers.

Radiography (London, England : 1995)·2023
Same author

Accuracy of radiographer preliminary clinical evaluation of skeletal trauma radiographs, in clinical practice at a district general hospital.

Radiography (London, England : 1995)·2022
Same author

Agreement in the reporting of General Practitioner requested musculoskeletal radiographs: Reporting radiographers and consultant radiologists compared with an index radiologist.

Radiography (London, England : 1995)·2022
Same author

Concordance between a neuroradiologist, a consultant radiologist and trained reporting radiographers interpreting MRI head examinations: An empirical study.

Radiography (London, England : 1995)·2020
Same author

Multicenter Postmarket Analysis of the Neuroform Atlas Stent for Stent-Assisted Coil Embolization of Intracranial Aneurysms.

AJNR. American journal of neuroradiology·2020
Same journal

Chronic hyperinsulinemia accelerates adipose senescence via mitochondrial dysfunction and cGAS-STING signalling.

The Journal of endocrinology·2026
Same journal

Probiotic Lactobacillus reuteri KUB-AC5 provides cardioprotection via mitigating cardiac mitochondrial dysfunction in obese rats.

The Journal of endocrinology·2026
Same journal

Relationship between hypothalamic astrocytes and gonadotropin-releasing hormone neurons in the female plains vizcacha.

The Journal of endocrinology·2026
Same journal

Passive exercise failed to reverse OVX-induced bone microstructural degeneration to the extent achieved by HRT.

The Journal of endocrinology·2026
Same journal

Downregulation of SLC32A1 mediates the PAI-1 pathway in alleviating hepatic stellate cell activation and liver fibrosis.

The Journal of endocrinology·2026
Same journal

Triiodothyronine-driven pro-inflammatory responses of dendritic cells are restrained by sphingolipid signaling.

The Journal of endocrinology·2026
See all related articles

Early human pancreas development involves PDX1 and CK19 expression in embryonic cells. Fetal beta cells mature into endocrine cells by the first trimester, expressing key hormones and markers.

Area of Science:

  • Developmental Biology
  • Endocrinology
  • Genetics

Background:

  • Human pancreas development studies are limited, often relying on rodent models.
  • Gene expression and cellular changes during early human pancreas formation are not fully understood.

Purpose of the Study:

  • To characterize gene expression and cellular differentiation during early human pancreas development.
  • To identify key markers and developmental milestones in human pancreatic beta cell maturation.

Main Methods:

  • Immunohistochemistry and immunofluorescence to detect protein expression (PDX1, CK19, insulin, glucagon, somatostatin, PP).
  • Analysis of pancreatic tissue at sequential embryonic and fetal stages (weeks post-conception).
  • Assessment of cellular proliferation and vascularization within developing pancreatic islets.

Related Experiment Videos

Main Results:

  • Embryonic pancreatic cells expressed PDX1 and CK19; later, fetal beta cells primarily expressed insulin.
  • CK19 expression decreased as insulin-positive cell clusters grew, with PDX1 remaining in fetal beta cells.
  • Vascularization became closely associated with insulin-positive cell clusters by 10 weeks post-conception, and mature islet markers were detected by 12-13 weeks.

Conclusions:

  • Fetal beta cells exhibit characteristics of true endocrine cells by the end of the first trimester.
  • The study provides a detailed timeline of molecular and cellular events in early human pancreas development.
  • Findings highlight the importance of vascularization in islet development and function.