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Updated: Aug 24, 2026

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
Published on: April 19, 2011
Isolation of cDNAs encoding typical and novel types of phosphoinositide-specific phospholipase C from the moss
Koji Mikami1, Alexander Repp, Elena Graebe-Abts
1National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki 444-8585, Japan. mikami@nibb.ac.jp
Abstract:
Two cDNAs encoding proteins, PpPLC1 and PpPLC2, with catalytic and C2 domains conserved in plant phosphoinositide-specific phospholipase C (PI-PLC) were isolated from Physcomitrella patens. The N domain, which has been identified in Arabidopsis PI-PLCs as an EF hand-like domain, was found in both isoforms, although that in PpPLC2 was a split type. At micromolar Ca2+ concentrations, PpPLC1 preferentially hydrolysed phosphatidylinositol-4,5-bisphosphate, while PpPLC2 showed no specificity. Furthermore, at millimolar Ca2+, phosphatidylinositol was hydrolysed by PpPLC2, but not by PpPLC1. Thus, PpPLC1 and PpPLC2 are typical and novel types of plant PI-PLC, respectively.
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