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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
[Groups and sources of yeasts in house dust]
A M Glushakova1, T M Zheltikova, I Iu Chernov
1Faculty of Soil Science, Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
Abstract:
House dust contains bacteria, mycelial fungi, microarthropods, and yeasts. The house dust samples collected in 25 apartments in Moscow and the Moscow region were found to contain yeasts belonging to the genera Candida, Cryptococcus, Debaryomyces, Rhodotorula, Sporobolomyces, and Trichosporon. The most frequently encountered microorganisms were typical epiphytic yeasts, such as Cryptococcus diffluens and Rhodotorula mucilaginosa, which are capable of long-term preservation in an inactive state. The direct source of epiphytic yeasts occurring in the house dust might be the indoor plants, which were contaminated with these yeasts, albeit to a lesser degree than outdoor plants. Along with the typical epiphytic yeasts, the house dust contained the opportunistic yeast pathogens Candida catenulata, C. guillermondii, C. haemulonii, C. rugosa, and C. tropicalis, which are known as the causal agents of candidiasis. We failed to reveal any correlation between the abundance of particular yeast species in the house dust, residential characteristics, and the atopic dermatitis of the inhabitants.
Insights
House dust harbors various yeasts, including opportunistic pathogens like Candida. Indoor plants may contribute to yeast presence, but no link was found to atopic dermatitis or home characteristics.
Area of Science:
- Microbiology
- Environmental Science
- Mycology
Context:
- House dust is a complex ecosystem containing diverse microorganisms.
- Yeasts are common inhabitants of indoor environments, including residential dust.
- Understanding yeast populations in homes is crucial for public health.
Purpose:
- To identify and characterize yeast species present in house dust samples from Moscow apartments.
- To investigate potential sources of yeast contamination, such as indoor plants.
- To explore correlations between yeast abundance, residential factors, and atopic dermatitis.
Summary:
- Analysis of house dust from 25 Moscow apartments revealed yeasts from genera including Candida, Cryptococcus, Debaryomyces, Rhodotorula, Sporobolomyces, and Trichosporon.
- Common epiphytic yeasts (Cryptococcus diffluens, Rhodotorula mucilaginosa) and opportunistic pathogens (Candida spp.) were identified.
- Indoor plants were suggested as a potential source for epiphytic yeasts, though less contaminated than outdoor plants.
Impact:
- Identifies common and pathogenic yeasts in residential dust, contributing to environmental microbiology data.
- Highlights the potential role of indoor plants in yeast distribution within homes.
- Provides baseline data for future studies on indoor microbial ecology and health implications, despite no correlation found with atopic dermatitis in this study.
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