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[Simple method for measurement of erythrocyte osmotic fragility]
H Ideguchi1, K Shinohara, A Ishikawa
1Department of Clinical Chemistry and Laboratory Medicine, Fukuoka University Hospital.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|May 1, 1992
Abstract:
We developed a simple and quantitative method for determination of erythrocyte osmotic fragility, which is based on a logarithmic linearization of osmotic fragility curve and a calculation of three parameters, that is, C50 (the osmolarity causing 50% hemolysis), beta (the slope of the curve) and H (% hemolysis before treatment). By making use of personal computer program, calculation of the parameters and print-out of reports are automated. As the results, it takes only 30 min to finish all procedure. This method is very useful particularly for the laboratory screening of hereditary spherocytosis.