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[Correction of haptoglobin contents in hemolytic specimen with addition of saturated hemoglobin]

T Okazaki1, H Arakawa

  • 11st Department of Mitsubishi Yuka Bio-Clinical Laboratories, Inc., Tokyo.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|May 1, 1992
PubMed

Measurement of serum haptoglobin by immunoturbidimetry was markedly interfered with hemolysis because of haptoglobin-hemoglobin complex formation. Turbidity formed with haptoglobin and immunoglobulin complexes was gradually decreased by the addition of hemoglobin in the reaction mixture, and reached to a certain fixed levels when serum haptoglobin were saturated with hemoglobin. The turbidity derived from saturated haptoglobin and hemoglobin complex closely correlated with the haptoglobin level without hemoglobin. Thus, we could measure the corrected haptoglobin level in hemolytic specimen by the saturation of hemoglobin in reaction mixture.

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