Related Experiment Video
Updated: Aug 24, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output
Ralf Paul1, Stefan Weiser, Nicholas C Amiot
1Division of Molecular Microbiology, Biozentrum, University of Basel, 4056 Basel, Switzerland.
Abstract:
Pole development is coordinated with the Caulobacter crescentus cell cycle by two-component signaling proteins. We show that an unusual response regulator, PleD, is required for polar differentiation and is sequestered to the cell pole only when it is activated by phosphorylation. Dynamic localization of PleD to the cell pole provides a mechanism to temporally and spatially control the signaling output of PleD during development. Targeting of PleD to the cell pole is coupled to the activation of a C-terminal guanylate cyclase domain, which catalyzes the synthesis of cyclic di-guanosine monophosphate. We propose that the local action of this novel-type guanylate cyclase might constitute a general regulatory principle in bacterial growth and development.
More Related Videos
12:24DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
09:40Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
Published on: September 20, 2011
Related Concept Videos
Global Regulatory Systems
Stringent Response in E. coli
Positive Regulator Molecules
Positive Regulator Molecules
Coordination of Gene Expression Processes in Bacteria
GPCRs Regulate Adenylyl Cylase Activity
Two...