Confidence interval estimation for DNA and mRNA concentration by real-time PCR: A new environment for an old theorem
P Verderio1, C Orlando, C Casini Raggi
1Operative Unit of Medical Statistics and Biometry, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy. paolo.verderio@istitutotumori.mi.it
The International Journal of Biological Markers
|April 14, 2004
Abstract
No abstract available in PubMed .
Related Concept Videos
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
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