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Related Experiment Videos

Platelet storage solution affects on the accuracy of laboratory tests for platelet function: a multi-laboratory

T VandenBroeke1, L J Dumont, S Hunter

  • 1Gambro BCT, Lakewood, CO, USA. tania.vandenbroeke@gambrobct.com

Vox Sanguinis
|April 14, 2004
PubMed
Summary

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Platelet function tests, including extent of shape change (ESC) and hypotonic shock response (HSR), are significantly impacted by the diluent used. For accurate in vitro platelet viability assessment, always use plasma, preferably autologous, for dilutions.

Area of Science:

  • Hematology
  • Transfusion Medicine
  • Platelet Physiology

Background:

  • Extent of shape change (ESC) and hypotonic shock response (HSR) are established in vitro assays for platelet function.
  • These assays traditionally use platelet-poor plasma (PPP) as the diluent.
  • The increasing use of synthetic storage media for platelets necessitates evaluating their impact on assay diluents.

Purpose of the Study:

  • To investigate the effect of using platelet storage solutions versus plasma as diluents for ESC and HSR assays.
  • To determine if storage media impacts the accuracy of in vitro platelet function testing.

Main Methods:

  • Six laboratories participated in a multi-center study.
  • Platelets were stored in either plasma or storage solution.

Related Experiment Videos

  • ESC and HSR were tested on days 1 and 5 using autologous plasma, fresh-frozen plasma, or storage solution as diluents.

    • Main Results:

    • Dilutions in storage solutions yielded significantly lower ESC and HSR results (over 30% reduction) compared to autologous plasma.
    • Dilutions in fresh-frozen plasma also showed reduced results (over 16% reduction) compared to liquid autologous plasma.
    • Both ESC and HSR measurements were significantly affected by the choice of diluent.

    Conclusions:

    • Test diluents significantly influence ESC and HSR assay outcomes.
    • Plasma, particularly autologous plasma, is the recommended diluent for in vitro ESC and HSR testing.
    • Results emphasize the importance of consistent diluent selection for reliable platelet function assessment, irrespective of storage media.