Cytokine response in lower extremity ischaemia/reperfusion

We studied the local and systemic concentrations of pro-inflammatory cytokines produced during acute lower extremity ischaemia and following reperfusion in 19 patients undergoing thromboembolectomy of the femoral artery. Blood samples were taken from the femoral vein (local response) and radial artery (systemic response) in the ischaemic (baseline) phase, and 2, 12 and 24 h post-reperfusion. Associated lung injury was measured by the alveolar-arterial (A-a) oxygen gradient and post-reperfusion femoral vein plasma concentrations of creatinine kinase and lactic dehydrogenase were also measured. Local and systemic concentrations of interleukin (IL)-2 receptor and IL-6 (but not IL-1 beta) increased significantly after reperfusion. IL-8 concentration increased significantly in the radial artery. The high and progressively increasing concentration of IL-6 in the femoral vein suggests local production. The parallel increase in creatinine kinase concentration and A-a oxygen gradient indicates that IL-6 plays an important role in acute arterial occlusion and reperfusion injury.