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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Published on: February 28, 2015

Estrogen receptor microarrays: subtype-selective ligand binding.

Sung Hoon Kim1, Anobel Tamrazi, Kathryn E Carlson

  • 1Department of Chemistry, University of Illinois, 600 South Matthews Avenue, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|April 15, 2004
PubMed
Summary

We developed a novel nuclear hormone receptor microarray for studying estrogen receptors (ERalpha-LBD and ERbeta-LBD). This high-throughput method efficiently assesses ligand binding and can be adapted for other receptors and coregulator recruitment.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Background:

  • Nuclear hormone receptors play critical roles in cellular processes.
  • Estrogen receptors (ERs) are key targets for understanding hormonal regulation and disease.
  • Developing high-throughput methods to study receptor-ligand interactions is essential.

Purpose of the Study:

  • To present the first nuclear hormone receptor microarray.
  • To illustrate its application using the ligand-binding domains of estrogen receptors alpha and beta (ERalpha-LBD and ERbeta-LBD).
  • To demonstrate its utility in studying ligand binding and subtype selectivity.

Main Methods:

  • Proteins (ERalpha-LBD and ERbeta-LBD) were printed and attached to aldehyde slides.
  • Attachment efficiency was analyzed based on ligand-bound status (agonists vs. antagonists/unliganded).
  • Ligand-binding activity was assessed using an estradiol-fluorophore conjugate in competitive binding assays.

Main Results:

  • Attachment efficiency to slides was significantly higher for unliganded or antagonist-bound ER-LBDs compared to agonist-bound forms, suggesting orientation-specific binding.
  • The immobilized ERs retained significant ligand-binding activity.
  • Specific and subtype-selective binding of ligands to ERalpha-LBD and ERbeta-LBD was successfully determined.

Conclusions:

  • A novel, high-throughput nuclear hormone receptor microarray technique has been established.
  • This method allows for efficient and selective assessment of ligand binding to estrogen receptors.
  • The platform is adaptable for studying other nuclear hormone receptors and coregulator recruitment.