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Updated: Jul 14, 2026

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Published on: March 4, 2017
Determination of protein-ligand association thermochemistry using variable-temperature nanoelectrospray mass
Rambod Daneshfar1, Elena N Kitova, John S Klassen
1Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Abstract:
A novel temperature-controlled nanoelectrospray (nanoES) device, interfaced with a Fourier transform ion cyclotron resonance mass spectrometer, is used to measure the association constants, Kassoc, for a series of protein-carbohydrate complexes at solution temperatures ranging from 5 to 40 degrees C. From a van't Hoff analysis of the Kassoc values, the enthalpies and entropies of association (DeltaHassoc, DeltaSassoc) at 25 degrees C are determined. The nanoES/mass spectrometry-derived thermodynamic parameters are in agreement with values previously determined by isothermal titration calorimetry.

