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Transgene integration in plants: poking or patching holes in promiscuous genomes?
David A Somers1, Irina Makarevitch
1Department of Agronomy and Plant Genetics, Plant Molecular Genetics Institute, University of Minnesota, 1991 Upper Buford Circle, St. Paul, Minnesota, USA. somers@biosci.cbs.umn.edu
Current Opinion in Biotechnology
|April 15, 2004
Abstract:
Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgene loci and investigations aimed at genetically dissecting transgene integration mechanism(s) have provided new insights into the process.