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Updated: Aug 24, 2026

Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
Rapid test for early detection of mycoplasma contamination of the continuous cell line J774.2
Martina Loudová1, Jakub Novosad
1Department of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, University Hospital, Charles University in Prague, Sokolska tr. 408, 500 05 Hradec Kralove, Czech Republic. mloudova@post.cz
Abstract:
The authors describe a rapid, simple and inexpensive method for the routine testing of mycoplasma contamination of the continuous mouse macrophage-like cell line J774.2 using specific anti-mouse monoclonal antibodies (antiCD14, antiCD80) and flow cytometry.
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