Related Experiment Video
Updated: Aug 24, 2026
Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
Published on: April 19, 2024
Determination of Ochratoxin A in small volumes of human blood serum
G Köller1, U Rolle-Kampczyk, I Lehmann
1Department of Human Exposure Research and Epidemiology, Centre for Environmental Research Leipzig-Halle, Permoserstr. 15, 04318 Leipzig, Germany. gabor.koeller@ufz.de
Abstract:
A new simple and rapid method for analysing Ochratoxin A (OTA) in small volumes of human blood serum using capillary zone electrophoresis coupled to laser-induced fluorescence is described. The clean-up procedure solely consists of a double extraction step. To improve the reproducibility of migration times and quantification, two internal standards were used. The limit of detection was 0.55 ng/ml, with a linear range of 1-100 ng/ml of OTA in spiked human blood serum. The method is used to rapidly screen suspected patients.
