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Growth factor-induced delayed early response genes.

A Lanahan1, J B Williams, L K Sanders

  • 1Howard Hughes Medical Institute, Baltimore, Maryland.

Molecular and Cellular Biology
|September 1, 1992
PubMed
Summary
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Researchers identified delayed early response genes in mouse 3T3 cells crucial for growth factor-induced DNA replication. These genes, activated after new protein synthesis, indicate a complex cellular response to growth factors.

Area of Science:

  • Cellular and Molecular Biology
  • Gene Regulation
  • Signal Transduction

Background:

  • Growth factors trigger sequential gene expression for long-term cellular responses.
  • Immediate-early genes are rapidly activated by growth factors within minutes.
  • Understanding delayed gene responses is key to deciphering growth factor signaling pathways.

Purpose of the Study:

  • To identify and characterize delayed early response genes activated hours before DNA replication.
  • To investigate the protein synthesis requirement for delayed early gene activation.
  • To analyze the novelty and known relationships of these delayed early genes.

Main Methods:

  • cDNA cloning to isolate genes activated after a delay.
  • Partial or complete sequencing of identified delayed early cDNAs.

Related Experiment Videos

  • Analysis of gene sequence homology to known sequences.

    • Main Results:

    • Identified genes activated hours before serum-induced DNA replication in 3T3 cells.
    • Confirmed that delayed early gene activation requires new protein synthesis.
    • Sequencing revealed 8 known gene-related sequences and 5 novel sequences, including those for HMGI(Y), HMGI-C, APRT, MIF-related protein, MIP family protein, and cyclin CYL1.

    Conclusions:

    • The delayed early gene response to growth factors in 3T3 cells is complex.
    • These delayed genes play a role in mediating long-term cellular responses to growth factors.
    • The findings expand our understanding of the intricate gene regulatory networks governing cell proliferation.