Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CREB binds to multiple loci on human chromosome 22.

Ghia Euskirchen1, Thomas E Royce, Paul Bertone

  • 1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8005, USA>

Molecular and Cellular Biology
|April 15, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol for massively parallel RNA assay combined with immunoprecipitation for high-throughput analysis of RNA-protein interactions in cells.

STAR protocols·2026
Same author

<i>Rroid2</i> regulates effector-to-memory CD8<sup>+</sup> T cell differentiation during infection in vivo.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Widespread specific intron retention events in nuclear RNA complexes identified by sedimentation analysis of pluripotent cellular extracts.

Genome research·2025
Same author

X-linked deletion of Crossfirre, Firre, and Dxz4 in vivo uncovers diverse phenotypes and combinatorial effects on autosomes.

Nature communications·2024
Same author

The temporal dynamics of lncRNA Firre-mediated epigenetic and transcriptional regulation.

Nature communications·2024
Same author

Biophysical characterization of high-confidence, small human proteins.

Biophysical reports·2024

Researchers mapped cyclic AMP-responsive element-binding protein (CREB) binding sites on human chromosome 22. This provides new insights into CREB

Area of Science:

  • Genomics
  • Molecular Biology
  • Neuroscience

Background:

  • Cyclic AMP-responsive element-binding protein (CREB) is a key transcription factor regulating neuronal function.
  • Global CREB binding distribution analysis was previously lacking.

Purpose of the Study:

  • To map the genome-wide binding distribution of CREB on a human chromosome.
  • To identify CREB target genes and regulatory regions.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) to isolate CREB-bound DNA.
  • Genomic DNA microarray hybridization to analyze binding sites on human chromosome 22.

Main Results:

  • Identified 215 CREB binding sites and 100 potential gene targets on chromosome 22.

Related Experiment Videos

  • Found CREB binding near genes involved in signal transduction and neuronal function.
  • Discovered that most CREB binding sites were not near gene 5' ends, but in introns and unannotated regions, some near novel transcriptionally active regions.
  • Observed that CREB targets often contained variant cyclic AMP response element (CRE) sequences.
  • Showed that forskolin treatment altered the expression of several CREB target genes, both inducing and repressing them.

    • Conclusions:

    • Provides the first global map of CREB binding on a human chromosome.
    • Reveals novel insights into CREB's regulatory mechanisms, including binding site location and target gene modulation.
    • Highlights the complex role of CREB in human neuronal function and development.