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Related Experiment Videos

[Corneal preservation at 31 degrees C--experimental study].

M Zemba, Veronica Bobeico, M Bratulescu

    Oftalmologia (Bucharest, Romania : 1990)
    |April 16, 2004
    PubMed
    Summary

    Corneal preservation in Inosol-like medium is optimal for 7-10 days, potentially up to 14 days. This study determined the minimum initial endothelial cell density required for successful corneal preservation.

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    Area of Science:

    • Ophthalmology
    • Corneal Science
    • Tissue Preservation

    Background:

    • The corneal endothelium is crucial for maintaining corneal clarity.
    • Effective preservation media and protocols are vital for successful corneal transplantation.
    • Understanding endothelial cell loss during preservation is key to optimizing graft survival.

    Purpose of the Study:

    • To determine the optimal preservation period for corneas in Inosol-like medium.
    • To evaluate endothelial cell loss in human and pig corneas over time.
    • To establish the minimum initial endothelial cell density required for viable corneal preservation.

    Main Methods:

    • Human and pig corneas were preserved in Inosol-like medium.
    • Specular microscopy was used to assess endothelial cell density.

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  • Corneas were examined at baseline and after 1, 4, 7, 10, and 14 days of preservation.

    • Main Results:

    • Human corneas showed a mean endothelial cell loss of 14.7% after 7 days and 23.4% after 14 days.
    • Pig corneas exhibited a mean cell loss of 8.8% after 7 days and 23% after 14 days.
    • A minimum initial endothelial cell density of 2400 cells/mm² was identified as necessary.

    Conclusions:

    • The optimal preservation period for corneas in Inosol-like medium is 7-10 days, extendable to 14 days.
    • Endothelial cell loss increases with preservation duration, reaching approximately 23% after 14 days.
    • A minimum initial endothelial cell density of 2400 cells/mm² is recommended for successful corneal preservation.