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Updated: Aug 24, 2026
A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe
Published on: June 12, 2012
[Decontamination of ocular globes--comparison of three methods]
Veronica Bobeico1, Aurora Cotea, M Zemba
1Clinica de Oftalmologie, Spitalul Clinic de Urgenta Militar Central dr. Carol Davila.
Purpose:
Determination of the success degree of the three decontamination methods for the purpose of choosing the decontamination method before the prelevation of the cornea for its best preservation.
Material And Method:
The studied decontamination methods were: 1. Immersion of the ocular globe in Betadina (polividon--iod) 2. Immersion of the ocular globe in Silver Nitrate 3. Washing of the ocular globe with Gentamicina. 30 animal eyes (pig) were used, 10 for each method. A bacteriological sample was prelevated from each ocular globe, before and after the decontamination. Afterwards the cornea was immersed in solution of Inosol type, following the indicator of the Inosol for 10 days.
Results:
In each conjunctival the microbial population was present before the decontamination. Positive samples existed after the decontamination for every method, microbial concentration being however greatly reduced in the first 2 samples. The contamination of the conservation environment occurred for a cornea in the case of Betadine, for 2 cornea in the case of Silver Nitrate and for 7 corneas in the case of Gentamicin.
Conclusions:
1. Decontamination with Gentamicin 33 mg/ml solution is inefficient. 2. The efficacy of Silver Nitrate and Betadine solution is similarly. 3. The best option is immersion of the globe in Betadine solution.
