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A multidrug efflux pump inhibitor reduces fluoroquinolone resistance in Pseudomonas aeruginosa isolates

Ahmet Yilmaz Coban1, Bora Ekinci, Belma Durupinar

  • 1Ondokuz Mayis University, Medical School, Department of Microbiology and Clinical Microbiology, Samsun, Turkey. ayilmazc@omu.edu.tr

Chemotherapy
|April 16, 2004
PubMed

Insights

Multidrug efflux pump inhibitor MC-207,110 significantly reduced fluoroquinolone (FQ) resistance in Pseudomonas aeruginosa. This finding suggests MC-207,110 could be a valuable addition to clinical protocols for combating FQ resistance.

Area of Science:

  • Microbiology
  • Pharmacology
  • Molecular Biology

Background:

  • Antibiotic resistance is a growing threat, particularly in Gram-negative bacteria.
  • Fluoroquinolone (FQ) resistance mechanisms include target mutations and active efflux.
  • Reduced porin expression is also implicated in FQ resistance.

Purpose of the Study:

  • To investigate the interaction between FQs (levofloxacin, ofloxacin, ciprofloxacin) and the multidrug efflux pump inhibitor MC-207,110.
  • To assess the efficacy of MC-207,110 in overcoming FQ resistance in Pseudomonas aeruginosa.

Main Methods:

  • Checkerboard assay was employed using Pseudomonas aeruginosa.
  • Tested FQs at concentrations ranging from 0.06-64 µg/ml.
  • Tested MC-207,110 at concentrations ranging from 4-128 µg/ml.

Main Results:

  • MC-207,110 significantly inhibited FQ resistance and decreased Minimum Inhibitory Concentrations (MICs) at concentrations of 16-128 µg/ml.
  • The inhibitory effect was observed across levofloxacin, ofloxacin, and ciprofloxacin.
  • No significant inhibition was noted at lower MC-207,110 concentrations (4 and 8 µg/ml).

Conclusions:

  • MC-207,110 effectively inhibits FQ resistance in Pseudomonas aeruginosa in vitro.
  • MC-207,110 demonstrates potential as a therapeutic agent to overcome FQ resistance.
  • Further clinical studies are warranted to explore the utility of MC-207,110 in treatment protocols.

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