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Related Experiment Videos

pH required to kill Enterococcus faecalis in vitro.

Christopher P McHugh1, Ping Zhang, Suzanne Michalek

  • 1Department of Endodontics and Pulp Biology, School of Dentistry, University of Alabama at Birmingham, 35294-0007, USA.

Journal of Endodontics
|April 16, 2004
PubMed
Summary

This study determined the lethal pH for Enterococcus faecalis, finding that pH 11.5 and above prevents bacterial growth. Alkaline environments above pH 11.0 significantly inhibit E. faecalis, crucial for understanding its survival in challenging conditions.

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Area of Science:

  • Microbiology
  • Environmental Science

Background:

  • Enterococcus faecalis is known for its resilience in alkaline conditions.
  • The precise pH threshold for inhibiting E. faecalis growth remains undefined.

Purpose of the Study:

  • To determine the minimum pH level that prevents Enterococcus faecalis growth.
  • To investigate the impact of increasing alkalinity on E. faecalis viability.

Main Methods:

  • Bacterial cultures of E. faecalis were incubated across a pH range from 9.5 to 12, with 0.5 increments.
  • Growth was assessed using turbidity, visual inspection, and spectrophotometry over 7 days.
  • Multiple replicates (12 tubes per pH level) were employed for robust data collection.

Main Results:

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  • E. faecalis exhibited growth at pH 9.5, 10, and 10.5 within 24-48 hours.
  • Significant growth retardation was observed between pH 10.5 and 11.0.
  • No growth was detected at pH 11.5 or pH 12, indicating a lethal threshold.

    • Conclusions:

    • A pH of 11.5 or greater is lethal to Enterococcus faecalis.
    • Alkalinity between pH 10.5 and 11.0 significantly impedes E. faecalis proliferation.
    • These findings provide critical data on the environmental limits of E. faecalis survival.