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Updated: Jul 24, 2026
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
Published on: June 15, 2012
Effect of laparoscopy versus laparotomy on circulating tumor cells using isolation by size of epithelial tumor cells
L Lelievre1, P Paterlini-Brechot, S Camatte
1Service de Chirurgie Gynécologique et Oncologique, Hôpital Européen Georges Pompidou, 20 rue Leblanc, 75015 Paris, France. lelievre.loic@wanadoo.fr
Aim:
To assess the effect of laparoscopy on circulating tumor cell (CTC) detection in case of carcinosis.
Material And Methods:
We compared laparoscopy versus laparotomy on tumor cell blood release in an animal model of ovarian carcinosis obtained by intraperitoneal inoculation of IGR-OV1 cells in nude rats. Animals were randomly assigned to one of the following groups: CO(2) laparoscopy (L), gasless laparoscopy (GL), midline laparotomy (ML), or general anesthesia as control (C). A 0.5 ml blood sample was taken in each case before and after experiment and tested with a novel assay, ISET (isolation by size of epithelial tumor cells), which isolates CTC by filtration on account of their size. Statistics were performed with the Fisher's and the Chi-square tests.
Results:
Ten rats were included in each group. We did not find any significant difference in CTC prevalence before and after surgery (2/14 versus 3/19, respectively, P = 1). Similarly, the three surgical accesses were equivalent with one post-experiment detection per group: 1/5 for L, 1/7 for ML, 1/7 for GL, and 1/6 for C (P = 0.9).
Conclusion:
This trial did not show any deleterious effect of laparoscopy on CTC when compared to laparotomy.
