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Updated: Aug 24, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Separation of conjugated linoleic acid methyl esters by silver-ion high performance liquid chromatography in
1Agricultural Research Service, Food Quality and Safety Research Unit, Fat & Industrial Oil Research, NCAUR, US Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA. adlofro@ncaur.usda.gov
Abstract:
Silver-ion HPLC (Ag-HPLC) has been utilized to separate a variety of unsaturated fatty acid methyl esters (FAMEs) by configuration, location or number of olefinic or acetylenic bonds. Two analytical Ag-HPLC columns connected in series and an isocratic solvent system of acetonitrile (ACN) in hexane were used to fractionate 10-15 mg samples of a mixture of two deuterium-labeled isomers of conjugated linoleic acid (Z9.E11- and Z9,Z11-octadecadienoic acid-17,17,18,18-d4). "Baseline" (> 95%) resolution of the two isomers, which decreased with increasing weights of sample injected, was maintained by careful adjustment of the percentage of ACN in the ACN/hexane solvent system. Chemical purities of the isolated FAME were > 96%.
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