Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Zebrafish: a preclinical model for drug screening.

Chuenlei Parng1, Wen Lin Seng, Carlos Semino

  • 1Phylonix, Cambridge, MA 02139, USA. chuenlei@phylonix.com

Assay and Drug Development Technologies
|April 20, 2004
PubMed
Summary

Zebrafish embryos offer a rapid, cost-effective vertebrate model for drug screening. These bioassays accurately assess toxicity, angiogenesis, and apoptosis, comparable to mouse models.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

uPAR-targeted stealth small-sized mesoporous silica nanoparticles for pancreatic cancer therapy.

Drug delivery and translational research·2026
Same author

Discovery of a Series of Pyrimidine Carboxamides as Inhibitors of Vanin-1.

Journal of medicinal chemistry·2021
Same author

Virtual consultations: Providing alternative ways of supporting patients with inherited bleeding disorders.

Haemophilia : the official journal of the World Federation of Hemophilia·2020
Same author

Investigating the clearance of VWF A-domains using site-directed PEGylation and novel N-linked glycosylation.

Journal of thrombosis and haemostasis : JTH·2020
Same author

Induction and Impact of Anti-Drug Responses Elicited by a Human Recombinant Coagulation Factor FXa<sup>I16L</sup> in Preclinical Species.

The AAPS journal·2019
Same author

Neutralizing Antibody Assay Development with High Drug and Target Tolerance to Support Clinical Development of an Anti-TFPI Therapeutic Monoclonal Antibody.

The AAPS journal·2019

Area of Science:

  • Developmental biology
  • Toxicology
  • Pharmacology

Background:

  • Zebrafish embryos are increasingly utilized as a vertebrate model for drug effect assessment.
  • Their transparency, ease of maintenance, and rapid life cycle facilitate genetic, embryological, and cell biology studies.
  • Zebrafish bioassays offer a faster and more economical alternative to traditional mouse assays for large-scale drug screening.

Purpose of the Study:

  • To describe the application of zebrafish bioassays for evaluating drug toxicity, angiogenesis, and apoptosis.
  • To demonstrate the comparability of zebrafish toxicity data with mouse models.
  • To showcase the utility of zebrafish assays for high-throughput screening.

Main Methods:

  • Toxicity and teratogenic effects were assessed using 18 chemicals, with results compared to mouse data.

Related Experiment Videos

  • Angiogenesis was evaluated using alkaline phosphatase staining and enzyme assays for compounds like SU5416 and flavopiridol.
  • Apoptosis was visualized and quantified using in vivo acridine orange staining and fluorescence microplate reading.

    • Main Results:

    • Zebrafish bioassays showed comparable toxicity, teratogenic effects, and LC50 values to mouse assays.
    • The transparency of zebrafish embryos allowed for direct observation of drug effects on organs like the heart, brain, and liver.
    • Antiangiogenic compounds SU5416 and flavopiridol were confirmed to inhibit blood vessel growth in zebrafish.
    • Acridine orange staining effectively visualized and quantified apoptotic events in treated zebrafish embryos.

    Conclusions:

    • Zebrafish embryos provide an efficient, cost-effective, and rapid vertebrate model for drug screening and assessment.
    • These bioassays are suitable for evaluating toxicity, angiogenesis, and apoptosis with high-throughput capabilities.
    • Zebrafish assays offer a valuable tool for understanding drug effects on various organs and biological processes.