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[Effect of testosterone on expression of androgen receptor in human monocytic cell line THP-1]
Chang-qing Liu1, Sai-zhu Wu, Zi-dong Wang
1Department of Cardiology, Nanfang Hostipal, First Military Medical University, Guangzhou 510515, China. liucqing@fimmu.com
Objective:
To investigate the effect of testosterone on the expression of androgen receptor (AR) in human monocytic cell line THP-1.
Methods:
Cultured THP-1 cells were stimulated by testosterone with different concentrations and the AR protein was examined by Western blotting.
Results:
AR expression was observed in unstimulated THP-1 cells and the quantity of AR expression was reduced by testosterone treatment in a dose-dependent manner. A selective AR blocker completely abolished this inhibitory effect of testosterone on AR expresison.
Conclusion:
Testosterone simulation time- and dose dependently reduces AR expression in THP-1 cells.
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