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Updated: Aug 24, 2026

Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice
Published on: May 5, 2011
Effects of ventilatory pattern on experimental lung injury caused by high airway pressure
Dana A Simonson1, Alexander B Adams, Laurel A Wright
1Department of Pulmonary and Critical Care Medicine, Regions Hospital/HealthPartners and University of Minnesota Medical School, USA.
Objective:
To determine the influence of clinician-adjustable ventilator settings on the development of ventilator-induced lung injury, as assessed by changes in gas exchange (Pao2), compliance, functional residual capacity, and wet weight to dry weight ratio.
Design:
Randomized in vivo rabbit study.
Setting:
Hospital research laboratory.
Subjects:
Forty-four anesthetized, mechanically ventilated adult rabbits.
Interventions:
Ventilation for 2 hrs with pressure control ventilation at 45 cm H2O, Fio2 = 0.6, and randomization to one of five ventilatory strategies using combinations of positive end-expiratory pressure (3 or 12 cm H2O), inspiratory time (0.45, 1.0, or 2.0 secs), and frequency (9 or 23/min).
Measurements And Main Results:
Among the ventilator strategies applied, PEEP at 12 cm H2O (elevated positive end-expiratory pressure) and inspiratory time at 0.45 secs (reduced inspiratory time) best preserved Pao2 (p <.003) and compliance (p <.035). During injury development, two consistent changes were observed: Tidal volume increased, and airway pressure waveform was transformed by extending the time to attain target pressure.
Conclusions:
In this preclinical model, lung injury was attenuated by decreasing inspiratory time. As lung injury occurred, tidal volume increased and airway pressure waveform changed.
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