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Indium detoxification in Pseudomonas fluorescens.

S Anderson1, V D Appanna

  • 1Department of Chemistry and Biochemistry, Laurentian University, Ramsey Lake Road, Sudbury, Ontario, Canada P3E 2C6.

Environmental Pollution (Barking, Essex : 1987)
|January 1, 1993
PubMed
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Soil bacteria Pseudomonas fluorescens can detoxify toxic indium by forming insoluble phosphorus compounds. Iron addition further aids indium homeostasis, suggesting potential for bioremediation of metal pollution.

Area of Science:

  • Environmental microbiology
  • Biogeochemistry
  • Toxicology

Background:

  • Indium is a non-essential toxic element with potential environmental impacts.
  • Soil bacteria play a crucial role in nutrient cycling and metal transformation in ecosystems.

Purpose of the Study:

  • To investigate the interaction between indium and the soil bacterium Pseudomonas fluorescens.
  • To understand the mechanisms of indium toxicity and tolerance in bacteria.
  • To explore the potential of bacterial metal detoxification for pollution management.

Main Methods:

  • Culturing Pseudomonas fluorescens in media with indium citrate.
  • Supplementing media with iron (III) to assess its effect on indium toxicity.
  • Analyzing bacterial growth rate and cellular yield.

Related Experiment Videos

  • Investigating bacterial responses using electrophoretic analysis of cytoplasmic extracts.
  • Measuring extracellular carbohydrate production.

    • Main Results:

    • Indium citrate inhibited bacterial growth and yield, but Pseudomonas fluorescens detoxified indium by forming insoluble phosphorus residues.
    • The addition of iron (III) mitigated indium's negative impact, leading to no reduction in cellular yield.
    • Indium homeostasis was achieved through the formation of extracellular phosphorus deposits.
    • Electrophoretic analysis revealed the induction of two polypeptides (57 kDa and 18 kDa) in metal-stressed bacteria.
    • Increased extracellular carbohydrates were observed in metal-supplemented media, with no detectable citrate in spent fluid.

    Conclusions:

    • Pseudomonas fluorescens possesses mechanisms to circumvent indium toxicity through insolubilization.
    • Iron (III) plays a role in enhancing bacterial tolerance and homeostasis to indium.
    • The observed bacterial responses, including phosphorus deposit formation, suggest potential applications in managing indium-induced metal pollution.