A review of enabling technologies based on scanning probe microscopy relevant to bioanalysis

Area of Science:

• Biophysics
• Nanotechnology
• Biomolecular Engineering

Background:

• Scanning probe microscopy (SPM) is a vital tool for in vitro biomolecular system analysis.
• SPM operates effectively in biocompatible fluids, enabling real-time monitoring of cellular and molecular dynamics.

Purpose of the Study:

• To highlight SPM's capabilities in imaging and force sensing at nanoscale and pN resolution.
• To explore SPM's potential in monitoring cellular responses to stimuli and pharmaceutical interventions.
• To review SPM's contributions to understanding biomolecular interactions and its application in novel bio-devices.

Main Methods:

• Utilizing SPM for non-intrusive interrogation of biomolecular systems.
• Employing SPM for real-time, real-space imaging at nm resolution.
• Leveraging SPM for pN force-sensing and force-imposing capabilities.

Main Results:

• SPM enables detailed observation of biomolecular dynamics in physiological environments.
• SPM facilitates the study of cellular responses to external factors.
• SPM aids in elucidating biomolecular interactions at surfaces and interfaces.

Conclusions:

• SPM is a versatile tool for biomolecular research, offering high-resolution imaging and force analysis.
• SPM holds promise for drug discovery and personalized medicine applications.
• SPM platforms are being reconfigured for advanced bio-device functionalities.