Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A biochip platform for cell transfection assays.

Endre Szili1, Helmut Thissen, Jason P Hayes

  • 1School of Chemistry, Physics and Earth Sciences, Flinders University of South Australia, Adelaide 5001, Australia.

Biosensors & Bioelectronics
|April 20, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Antimicrobial Peptides via Motif Assembly for Combating Multidrug-Resistant Pathogens.

Journal of medicinal chemistry·2026
Same author

Imprinted Binary Colloidal Crystals Support Growth and Stemness of Mouse Embryonic Stem Cells.

ACS biomaterials science & engineering·2025
Same author

Machine Learning-Assisted Prediction and Generation of Antimicrobial Peptides.

Small science·2025
Same author

Cold-Spray Deposition of Antibacterial Molybdenum Coatings on Poly(dimethylsiloxane).

ACS applied bio materials·2025
Same author

Critical updates on oral insulin drug delivery systems for type 2 diabetes mellitus.

Journal of nanobiotechnology·2025
Same author

Clinical challenges and opportunities related to the biological responses experienced by indwelling and implantable bioelectronic medical devices.

Acta biomaterialia·2024

This study presents a novel biochip for controlled cell adhesion and transfection. The platform enables targeted cell manipulation and gene delivery using laser patterning for enhanced transfection efficiency.

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Surface Science and Materials Chemistry
  • Cell Biology and Genetic Engineering

Background:

  • Cell transfection assays require precise control over cell adhesion and localization.
  • Existing methods for cell patterning and manipulation can be complex and lack spatial resolution.
  • Surface modification techniques are crucial for developing advanced biochip platforms.

Purpose of the Study:

  • To develop and characterize a novel biochip platform for spatially controlled cell transfection assays.
  • To investigate the use of surface modification and laser ablation for creating patterned biointerfaces.
  • To evaluate the efficiency of cell attachment and gene transfection on the developed biochip.

Main Methods:

  • Surface modification of silicon wafers using allylamine plasma polymer (ALAPP) and poly(ethylene oxide) (PEO) grafting.

Related Experiment Videos

  • Excimer laser ablation for patterning the ALAPP-PEO coated surfaces to control protein adsorption and cell attachment.
  • X-ray photoelectron spectroscopy (XPS) for surface characterization.
  • Cell culture experiments with human embryonic kidney (HEK 293) cells and plasmid DNA transfection (GFP).

    • Main Results:

    • Successful creation of a two-dimensionally controlled surface chemistry on the biochip.
    • Human embryonic kidney cells exclusively attached to laser-ablated areas.
    • Cells confined to ablated areas were successfully transfected with plasmid DNA encoding green fluorescent protein (GFP).
    • Transfection efficiencies were 21% for cells in culture flasks and 13% for cells on the biochip.

    Conclusions:

    • The developed biochip platform enables precise spatial control over cell attachment.
    • Laser-patterned surfaces facilitate targeted cell adhesion and subsequent gene transfection.
    • This technology holds promise for advanced cell-based assays and targeted genetic manipulation.