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Related Experiment Videos

Segmented flow generation by chip reactors for highly parallelized cell cultivation.

Andreas Grodrian1, Josef Metze, Thomas Henkel

  • 1Institute for Bioprocessing and Analytical Measurement Techniques, Heiligenstadt, Germany.

Biosensors & Bioelectronics
|April 20, 2004
PubMed
Summary
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This study presents a microfluidic system for rapid cell suspension aliquot generation using segmented flow. The technology enables high-throughput analysis and retrieval of selected clones from microorganisms.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Cell Biology

Background:

  • Micro system technology provides tools for handling small liquid volumes in cell cultivation.
  • Existing methods may face challenges with compatibility between cell surfaces and microfluidic device walls.
  • High-throughput analysis of cell cultures requires efficient methods for generating and isolating individual culture volumes.

Purpose of the Study:

  • To present a modular microfluidic system for the rapid generation of cell suspension aliquots.
  • To enable the production and analysis of large numbers of well-separated culture volumes.
  • To demonstrate the retrieval of selected clones from the generated micro-cultures.

Main Methods:

  • Application of the segmented flow principle using non-miscible liquids (alkanes) to separate aqueous culture medium droplets.

Related Experiment Videos

  • Utilizing micro flow transportation within chip channels and chamber topologies for droplet guidance.
  • Employing alkanes to prevent direct contact between cell walls and channel surfaces, avoiding compatibility issues.

    • Main Results:

    • The system successfully generates and separates individual cell culture droplets at frequencies up to 30 Hz per microchannel.
    • This results in the production of approximately 10^5 individual culture volumes per hour, or 2 million per day.
    • Demonstrated survival and growth of microorganisms, including model organisms and those from a natural soil sample.

    Conclusions:

    • The developed microfluidic system offers a serially operating cell processing solution for high-throughput cell cultivation.
    • Segmented flow in microfluidics effectively prevents wall-related compatibility issues, enhancing cell viability.
    • This technology facilitates rapid generation, analysis, and potential retrieval of specific clones from microbial populations.